The clinical gene amplification experiment, also known as PCR experiment, is a detection method specially used to test for viral infectious diseases such as AIDS, Hepatitis B and Avian Epidemic Disease. It can be used to amplify the genes contained in the virus to detect whether some infected people with low virus content contain specific viruses. It can also detect whether the CUVID-19 is in the human body.
2. The PCR laboratory layout
In principle, the clinical gene amplification testing laboratory is divided into four separate working areas: reagent storage and preparation area, specimen preparation area, amplification reaction mixture configuration and amplification area, and amplification product analysis area. In order to avoid cross-contamination, entering each work area must strictly follow a single direction, that is, only from the reagent storage and preparation area-specimen preparation area-amplification reaction mixture configuration and amplification area-amplification product analysis area.
3. Design and pressure control of laboratory air conditioning and ventilation system
The PCR laboratory does not have strict purification requirements, but in order to avoid the possibility of cross-contamination between the various experimental areas, it is advisable to adopt a full-flow and full-row airflow organization form. At the same time, we must strictly control the proportion of air supply and exhaust to ensure the pressure requirements of each experimental area.
4. Prevention and control of pollution
The core problem of PCR laboratory design is how to avoid contamination. In practical work, the following types of pollution are common: pollution of amplification products; pollution of natural genomic DNA; pollution of reagents and contamination between specimens. Because once the pollution occurs, the experiment must be stopped until the source of the pollution is found, and the result of the experiment must be invalidated, and the experiment must be repeated. Therefore, finding the source of pollution around the laboratory after pollution occurs is not only time-consuming but also cumbersome, and wastes manpower and material resources. Therefore, to avoid pollution, prevention should be the first step, not elimination.
5. Complete laboratory supporting facilities
Complete laboratory supporting facilities are necessary conditions to ensure the experimental work, and should be equipped with corresponding equipment and instruments according to the different experimental content of each laboratory, such as ultra-clean workbench, centrifuge, sample adder, etc.